Specifications Source Recombinant Human PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln31-Gln692.[Accession | Q8NBP7-1] Molecular Weight Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Human PCSK9, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA . SPR Data :Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). Formulation Supplied as 0.22μm filtered solution in 20mM PB, 300mM NaCl, 10% Glycerol (pH 7.4). Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.
