Specifications Synonyms alpha 1 beta 1;Integrin alpha 1 beta 1; alpha 1 Source Human Integrin alpha 1 beta 1 (ITGA1&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe29-Pro1141(ITGA1) acidic tail & Gln21-Asp728(ITGB1) basic tail.[Accession | P56199-1(ITGA1)&P05556-1(ITGB1)] Molecular Weight The protein has a predicted MW of 129.1 kDa(ITGA1)&83.2 kDa(ITGB1). Due to glycosylation, the protein migrates to 160-200 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Formulation Lyophilized from 0.22μm filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.