Specifications Source Human IL-23R Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Gly24-Gly355.[Accession | Q5VWK5-1] Molecular Weight The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result. Endotoxin < 1.0 EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Human IL-23 alpha&Mouse IL-12, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.88μg/ml determined by ELISA SPR Data: Human IL-23R, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 11.09 nM as determined in SPR assay (Biacore T200). Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only
