Specifications Synonyms GARP;LRRC32; LAP Source Human GARP(Y137H&S138G&G139N)&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains His20-Leu628 (GARP(Y137H&S138G&G139N)) & Leu30-Ser390 (Latent TGF beta 1).[Accession | Q14392(GARP(Y137H&S138G&G139N))&P01137(Latent TGF beta 1)] Molecular Weight The protein has a predicted MW of 70.3 kDa (GARP(Y137H&S138G&G139N))&41.4 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 75-80 kDa (GARP(Y137H&S138G&G139N))&15 kDa&43-47 kDa (Latent TGF Beta 1) based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.
