Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo . Mature human ANGPTL4 shares 26%-30% amino acid (aa) sequence identity with ANGPTL1, 2, 3, 5, 6, and 7. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers . This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels. In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL. In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL. Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity. Its expression in adipose tissue is induced by fasting and suppressed by feeding. In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells. The N-terminal fragment can function as an angiogenesis inhibitor. In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing. ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells . The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome. Specifications Synonyms ANGPTL4; ARP4; FIAF; HFARP; Angiopoietin like 4; NGPTL2; NL2; PGAR; pp1158; ANG-3; ANG4; AGP4; ANG3; ANG-3; ANG-4; Uniprot No. Q9BY76.1 Source Recombinant Human ANGPTL4/Angiopoietin-like 4 Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Pro166-Ser406. Molecular Weight Approximately 28.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human LILRB2 captured on Protein A chip, can bind Human ANGPTL4, His Tag with an affinity constant of 13.8nM as determined in a SPR assay (Biacore T200). Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transported with ice packs. Store at -20℃ to -80℃, valid for one year. After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days. It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data
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