Recombinant Biotinylated Human IL-13 Protein, His-Avi Tag

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Specifications Synonyms interleukin 13; IL-13;IL13; P600 Source Biotinylated Human IL-13 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Gl y21-Asn132.[Accession | AAK53823] Molecular Weight The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 90% as determined by SDS-PAGE Activity ELISA Data: Immobilized Biotinylated Human IL-13, His Tag at 2μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 1.15μg/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only. Show more