Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex. The Klotho beta mediated interaction of human FGF19 with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase and sterol 12-alpha hydroxylase Specifications Synonyms betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; β-klotho; klotho β like; Klotho β Uniprot No. Q86Z14 Source HEK293 Cells-derived Human Beta Klotho Protein, Met30-Thr983 with His tag at the C-terminal Molecular Weight Approximately 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage The products are shipped with ice pack and can be stored at -20℃ for 1 year. 2-7 days, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. Note 1.For your safety and health, please wear lab coats and disposable gloves for operation. 2.For research use only! Product Data
