Glypican 1, also known as GPC1, is a cell surface heparan sulfate proteoglycan that plays a crucial role in cell growth and development. It is a member of the glypican family, which are glycosylphosphatidylinositol-anchored proteins found on the cell surface. Specifications Synonyms Glypican 1, GPC1 Uniprot No. P35052-1 Source Recombinant Human Glypican 1/GPC1 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Asp24-Ser530. Molecular Weight The protein has a predicted MW of 57.17 kDa. And it migrates as 60-72 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Purity > 90% as determined by SDS-PAGE. Biological Activity Human FGF-2 immobilized on CM5 chip can bind human GPC1, His tag, with an affinity constant of 5.83 × 10-9 M as determined in a SPR assay (Biacore 8K). Endotoxin < 1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS. Reconstitution Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Note 1.Please operate with lab coats and disposable gloves, for your safety. 2.This product is for research use only. Product Data
