The LDL receptor-related proteins LRP-5 and LRP-6 constitute a distinct subgroup of the LDL receptor family. Both are widely expressed type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins in the beta-catenin signaling pathway. Both also play critical roles in gastrulation and skeletal patterning during development.LRP-5/6 associates with Wnt/Frizzled complexes, triggering phosphorylation of its intracellular PPPS motifs, binding of Axin, and propagation of beta -catenin signals. Specifications Synonyms LRP-5; LRP-7; LR3; BMND1; BMND1OPTA1; EVR1; EVR4; HBM; LR3VBCH2; LRP5; LRP7; OPPG; OPS; OPTA1; VBCH2 Uniprot No. O75197-1 Source Recombinant Human LRP-5 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Glu644-Gln1263. Molecular Weight The protein has a predicted MW of 96.51 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The products are shipped with dry ice and can be stored at -25~-15℃ for 1 year. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. Note 1. For your safety and health, please wear lab coats and disposable gloves for operation. 2. For research use only! Product Data
