Recombinant Biotinylated Human TLR3 Protein (Primary Amine Labeling), His Tag

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Synonyms IIAE2;TLR3; Source Biotinylated Human TLR3 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus. It contains Ser23-Glu703.[Accession | Q6PCD4] Molecular Weight The protein has a predicted MW of 78.7 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC Activity ELISA Data: Immobilized Biotinylated Human TLR3, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Storage The product should be stored at -85~-65℃ for 1 year from date of receipt. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only. Show more