Lymphotoxin beta receptor (LTBR), previously called TNF RIII or TNF R‑related protein (TNF Rrp), is a type I transmembrane glycoprotein within the TNF receptor superfamily, designated TNFRSF3. Components of LTBR-associated signaling complexes, including TRAF2, TRAF3, NIK, IKK1, and IKK2 have been shown to participate in the coupling of LTBR to NFkappaB. Product Information Synonyms TNFCR, TNFR3, TNFRSF3, LTBR, CD18, TNFR2-RP, TNFR-RP, LT-BETA-R, TNF-R-III, D12S370, LT-β-R Species Human Expression Region Gln31-Met227 Accession P36941-1 Tag C-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based onSDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin Level < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Bioactivity 【ELISA Data】Immobilized Human LIGHT, His Tag at 1 μg/ml (100 μl/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 13.8 ng/ml determined by ELISA . Immobilized Human LTBR, hFc Tag at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-LTBR Antibody, hFc Tag with the EC50 20.7 ng/ml determined by ELISA. Reconstitution Before opening, briefly centrifuge the vial.Reconstitute the lyophilized protein using sterile water to a concentration greater than 100 μg/mL.It is recommended to aliquot the reconstituted protein to avoid repeated freeze–thaw cycles. Product Features High purity High bioactivity Low endotoxin Excellent batch-to-batch consistency Application Storage Store at -25 °C to -15 °C with a shelf life of 2 years upon receipt.After reconstitution, store under sterile conditions at -85 °C to -65 °C for up to 3 months.
