Synonyms TGF beta1; DPD1;CEDLAP; TGFB1 Source Biotinylated Human LAP (TGF beta 1) Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Leu30-Arg278(C33S).[Accession | P01137] Molecular Weight The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.86μg/ml determined by ELISA .Immobilized Biotinylated Human LAP (TGF beta 1) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 23.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.
