Product and properties Synonyms TALL-2; TALL2;CD256; APRIL Source Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus. It contains Lys112-Leu250.[Accession | A0A2K5TJA1] Molecular Weight The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in 50mM Tris, 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
