Product properties Synonyms MASP1P1; MASP-2;MAP19; Source Cynomolgus MASP2 Protein is expressed from E.coli with His tag at the C-Terminus. It contains Thr287-Phe686.[Accession | A0A2K5UJY0] Molecular Weight The protein has a predicted MW of 46.8 kDa. Due to autocatalytic cleavage, the protein migrates to 21-24 kDa&28-30 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Cynomolgus MASP2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
