Human Alkaline Phosphatase (Germ type)/ALPG Protein, His Tag

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Product Properties Synonyms Alkaline phosphatase, placental-like;PLAP-like;ALP-1;Alkaline Phosphatase Source Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ile20-Asp503.[Accession | P10696] Molecular Weight The protein has a predicted MW of 53.67 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human Alkaline Phosphatase (Germ type) , His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage 1.The product should be stored at -25~-15℃ for 1 year from date of receipt. 2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution. 4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Show more