Product Properties Synonyms APOE;APOE3;Apo-E;Apolipoprotein E Source Human APOE3/Apolipoprotein E Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Lys19-His317.[Accession | P02649] Molecular Weight The protein has a predicted MW of 59.76 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity SPR Data:Human TREM2, His Tag captured on CM5 Chip via anti-his antibody can bind Human APOE3, hFc Tag with an affinity constant of 0.41 μM as determined in SPR assay . Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage 1.The product should be stored at -25~-15℃ for 1 year from date of receipt. 2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution. 4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
