Product Properties Synonyms CD117;c-kit;PBT;SCFR Source Human CD117 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln26-Pro520.[Accession | P10721-2] Molecular Weight The protein has a predicted MW of 82.46 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human CD117, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD117 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA .ELISA Data: Immobilized Human SCF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage 1.The product should be stored at -25~-15℃ for 1 year from date of receipt. 2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution. 4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
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