Product Properties Property Name Property Details Alternative Names notch ligand DLL4, Delta-like 4, MGC126344, DLL4 Species Human Expression Region Ser27–Pro524 Accession KT340-D Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 55.38 kDa. Due to glycosylation, the protein migrates to 56–70 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE, > 90% as determined by HPLC Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Bioactivity [ELISA Data] Immobilized Human DLL4, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 11.8 ng/mL determined by ELISA. Reconstitution Centrifuge the vial before opening. Reconstitute lyophilized protein using distilled water at a concentration above 100 μg/mL. It is recommended to aliquot the reconstituted protein for single use to avoid repeated freeze-thaw cycles. Features High purity, high bioactivity, low endotoxin, excellent lot-to-lot consistency Storage Store at -85°C to -65°C, valid for 1 year upon receipt. After reconstitution, store under sterile conditions at -85°C to -65°C, valid for 3 months.
