Product Properties Alternative Names ST2,FIT-1,DER4,IL1RL1 Species Human Expression Sequence Lys19-Phe328 Accession Q01638-2 Tag C-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 61.75 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human IL-33, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-1RL1, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
