Product Propertie Alternative Names Integrin alpha V beta 1,αVβ1,ITGAV&ITGB1 Species Human Expression Sequence Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail Accession P06756-1(ITGAV)&P05556-1(ITGB1) Tag C-His-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 130-160 kDa (ITGAV) and 100-120 kDa (ITGB1) based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human ITGAV&ITGB1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
