Product Propertie Alternative Names beta3,ITGAV&ITGB3Integrin alpha V beta 3,αVβ3 Species Human Expression Sequence Phe31-Val992(ITGAV) acidic tail&GLy27-Asp718(ITGB3) basic tail Accession P06756-1(ITGAV)&P05106-1(ITGB3) Tag C-His-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV)&81.3 kDa (ITGB3). Due to glycosylation,the protein migrates to 85-140 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human ITGAV&ITGB3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
