Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein, His-Avi Tag

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Product Propertie Alternative Names ITGAV&ITGB6Integrin alpha V beta 6,αVβ6 Species Human Expression Sequence Phe31-Val992(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail Acces sion P06756-1(ITGAV)&P18564-1(ITGB6) Tag C-His-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV)&79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-150 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.49μg/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months. Show more