Product Propertie Alternative Names NGN,neogenin 1,IGDCC2,NEO1 Species Human Expression Sequence Ala34-Leu1105 Accession Q92859-1 Tag C-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 144.37 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human RGMa, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
