Product Propertie Alternative Names OSMR,GP130 Species Human Expression Sequence Glu28-Met740(OSMR)&Glu23-Glu619(GP130) Accession Q99650-1(OSMR)&P40189-1(GP130) Tag C-His,C-Flag Expression System HEK293 Molecular Weight The protein has a predicted MW of 82.2 kDa(OSMR) and 68.83 kDa(GP130). Due to glycosylation, the protein migrates to 100-140 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human OSMR&GP130, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
