Human Siglec-10 (R119A) Protein, His-Avi Tag

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Product Propertie Alternative Names SLG2, SIGLEC10, MGC126774, PRO940 Species Human Expression Sequence Met17-Thr546 Accession Q96LC7-1 Tag C-His-Avi Expression System HEK293 Molec ular Weight The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris,150mM NaCl, 200mM Arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human Siglec-10 (R119A) , His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-10 (R119A) Antibody, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months. Show more