Product Propertie Alternative Names TL1A, VEGI-251, TNFSF15, TL1, VEGI, VEGI192A Species Human Expression Sequence Leu72-Leu251 Accession O95150-1 Tag N-monomeric hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 44.87 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE,> 90% as determined by HPLC Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Mouse DR3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFSF15, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA . 【ELISA Data】 Immobilized Human TNFSF15, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
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