Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His-Avi Tag

Yeasen - Leading Innovation in Molecular Enzymes and Reagents Verified independent store

→

Product Properties Alternative Names Integrin alpha 5 beta 1, VLA-5, ITGA5&ITGB1 Integrin alpha 5 β 1, α5β1 Species Human Expression Sequence Phe42-Tyr995(ITGA5) acidic tail&Gln21- Asp728(ITGB1) basic tail Accession P08648(ITGA5)&P05556-1(ITGB1) Tag C-His-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE,> 90% as determined by HPLC Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized 0.5μg Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months. Show more