CD23 is a type 2 transmembrane C-type lectin that binds IgE, CD21, CD11b and CD11c. It is expressed on a variety of hematopoietic cells and functions in allergic immune responses. Recombinant human sCD23 is a 19.2 kDa non-glycosylated protein containing 172 amino-acid residues. Product Properties Alternative Names FCER2 Species Human Expression Sequence Met1-Ser321 Accession P06734 Tag No Tag Expression System E.coli Molecular Weight Approximately 19.2 kDa. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 0.1 EU per µg by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Appearance Lyophilized Powder Biological Activity The ED50determined by its ability to induce TNF-alpha production by human PBMCs. Fully biologically active when compared to standard. Reconstitution Method Before opening the lid, centrifuge to bring the contents to the bottom. Reconstitute with sterile distilled water or PBS and reconstitute to a concentration of 0.1-1.0 mg/mL. It can be further diluted according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer. Aliquot into the amount for one experiment and freeze at -80℃ to avoid repeated freezing and thawing. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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