Product Properties Alternative Names IL23 alpha, IL12 beta, IL23 alpha&IL12 beta , IL12 β, IL23 alpha&IL12 β Species Human,Mouse Expression Sequence Arg20-Pro189 (Human IL-23 alpha) and Met23-Ser335 (Mouse IL-12 beta) Accession Q9NPF7(Human IL-23 alpha)&P43432-1(Mouse IL-12 beta) Tag C-His-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22-25 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Biotinylated Human IL-23 alpha & Mouse IL-12 beta, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 28.1ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -85 ~ -65℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
