Cynomolgus CA9/Carbonic Anhydrase IX Protein, His Tag

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Product Properties Alternative Names CA9, CAIX, CA-IX, EC 4.2.1.1, G250, MN, P54,58N, PMW1, RCC Species Cynomolgus Expression Sequence Gln38-Asp402 Accession A0A2K5VQG9 Tag C-His E xpression System HEK293 Molecular Weight The protein has a predicted MW of 41.03 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Cynomolgus CA9, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months. Show more