Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein, His-Flag Tag

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Product Properties Alternative Names CD3, CD3e, CD3E, CD3d, T3D, CD3D, CD3E&CD3D CD3 delta&CD3 epsilon Species Cynomolgus Expression Sequence Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D ) Accession Q95LI5.2(CD3E)&Q95LI8(CD3D) Tag C-His-Flag Expression System HEK293 Molecular Weight The protein has a predicted MW of 11.93 kDa (CD3E) and 10.55 kDa (CD3D). Due to glycosylation, the protein migrates to 13-20 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Cynomolgus CD3E&CD3D, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months. Show more