Product Properties Alternative Names CD83, HB15, BL11, CD83 molecule Species Cynomolgus Expression Sequence Thr20-Glu147 Accession A0A2K5US85 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 15.43 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Cynomolgus CD83, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
