Product Properties Alternative Names Interleukin-10, IL-10, CSIF, IL10A, TGIF, B-TCGF, GVHDS, MGC126450, MGC126451 Species Cynomolgus Expression Sequence Ser19-Asn178 Accession G7NVB0 Tag N-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 19.78 kDa. Due to glycosylation, the protein migrates to 20-22 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity ELISA Data: Immobilized Cynomolgus IL-10, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA . Reconstitution Method Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After resuspension, store at -85 to -65°C. The product is valid for three months.
