Product Properties Alternative Names CD218a, IL18Ra, IL18R1, IL1RRP, IL-1 R5, IL-1 Rrp, IL-18 R alpha, IL-18 R1, IL-18Ra Species Cynomolgus Expression Sequence Cys22-Gly329 Accession A0A2K5V3J9 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 36.45 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【SPR Data】Cynomolgus IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.02 nM as determined in SPR assay . Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.