Product Properties Alternative Names IFN-beta-2, IL6, IL-6, BSF-2, CDF, MGI-2A, Interleukin-6, HSF, IFNB2, HGF,IFN-β-2 Species Cynomolgus Expression Sequence Ala28-Met212 Accession P79341 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 22.06 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity ELISA Data: Immobilized Cynomolgus IL-6, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA. Reconstitution Method Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After resuspension, store at -85 to -65°C. The product is valid for three months.
