Product Properties Alternative Names αVβ3, Integrin alpha V beta 3, beta3, alphaV, ITGAV&ITGB3,Integrin alpha V β 3, β3 Species Cynomolgus Expression Sequence Phe31-Pro993(ITGAV) acidic tail & Gly27-Asp718(ITGB3) basic tail Accession A0A2K5WCD3(ITGAV)&A0A2K5WYN9(ITGB3) Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 112.31 kDa (ITGAV) & 81.27 kDa (ITGB3). Due to glycosylation, the protein migrates 95-140 to kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】Immobilized Cynomolgus ITGAV&ITGB3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-alpha V beta 3 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
