Cynomolgus TNFR1/CD120a/TNFRSF1A Protein, His Tag

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Product Properties Alternative Names TNF-R1, TNF-RI, FPF, TBP1, TNF-R55, TNFAR, TNFR60, p55, p60, MS5, p55-R, TNFR1-d2 Species Cynomolgus Expression Sequence Leu30-Thr211 Accession A0A7N9CVF2 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 21.62 kDa. Due to glycosylation, the protein migrates to 32-42 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Cynomolgus TNFR1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 17.2ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months. Show more