Product Properties Alternative Names IL-10RA, IL10RA, IL10R, IL-10R1, IL-10R subunit 1, CDw210a, HIL-10R, IBD28, IL-10 R alpha Species Cynomolgus Expression Sequence His22-Asn233 Accession A0A2K5WJ66 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 25.34 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-10 R alpha, His Tag with the EC50 of 49.8ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
