Product Properties Alternative Names HLA G antigen, sHLA-G, b2 microglobulin, HLA G, HLAG, HLA-G, MHC-G, sHLA-G Species Cynomolgus Expression Sequence Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide Accession E0WKX9(HLA-G)&Q8SPW0(B2M)&RIIPRHLQL Tag C-His-Avi Expression System HEK293 Molecular Weight The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【SPR Data】Cynomolgus LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with an affinity constant of 0.13 μM as determined in SPR assay . Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
