Product Properties Alternative Names C3B,C4B inactivator, CFI, IF, C3BINA, FIC3b-INA, I factor (complement), KAF Species Mouse Expression Sequence Arg19-Val603 Accession Q61129 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 66.39 kDa. Due to autocatalytic cleavage and glycosylation, the protein migrates to 80-100 kDa on SDS-PAGE result. Purity > 90% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in 50mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.