Product Properties Alternative Names MET, oncogene MET, HGF R, HGF,SF receptor, AUTS9, cMET, Met (c-Met), RCCP2, SF receptor Species Mouse Expression Sequence Glu25-Ala931 Accession P16056 Tag C-His Expression System HEK293 Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.1 kDa (α chain) and 69.8 kDa (β chain). Due to glycosylation, the protein migrates to 40-50 kDa (α subunit) and 78-98 kDa (β subunit) based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Mouse HGF R, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.
