Mouse RANKL/TNFSF11/CD254 Protein, His Tag

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Product Properties Alternative Names ODF, OPGL, RANKL, TRANCE, CD254, sOdf, TNFSF11, OPTB2 Species Mouse Expression Sequence Tyr70-Asp316 Accession AAC40113.1 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 28.97 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity ELISA Data: Immobilized Mouse RANKL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After resuspension, store at -85 to -65°C. The product is valid for three months. Show more