Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein

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Product Properties Alternative Names BMP-2, BMP-2A, BMP2A, BDA2, SSFSC, Species Human,Rhesus macaque,Mouse,Rat,Canine Expression Sequence Gln283-Arg396 Accession P12643 Tag No Tag Expression System E.coli Molecular Weight The protein has a predicted MW of 12.90 kDa same as SDS-PAGE result. Purity > 95% as determined by SDS-PAGE. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in 100mM Acetic Acid (pH 2.8). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【Cell Based Assay】Measured by its ability to induce Alkaline Phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 40-200 ng/mL . Reconstitution Method Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 100mM Acetic Acid (pH 2.8). Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months. Show more