Product Properties Alternative Names Alkaline Phosphatase, ALP-1, Alkaline phosphatase, placental-like, GCAP, PLAP-like, ALPG, ALPPL, ALPPL2 Species Rat Expression Sequence Val22-Pro510 Accession F1M8U7 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 54.10 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.
