Product Properties Alternative Names PAI, PAI-1, Serpin E1, SERPINE1, PAI1, PLANH1 Species Rat Expression Sequence Ser24-Pro402 Accession P20961 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 43.73 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 300 mM NaCl, 1 mM TCEP (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.
