Recombinant Cynomolgus HGF R/c-MET Protein, His Tag, Ultra Low Endotoxin _ 96264ES

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c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene .The protein possesses tyrosine k inase activity. Specifications Cat.No. 96264ES25 / 96264ES60 / 96264ES76 Size 25 μg / 100 μg / 500 μg Components Name 96264ES25 96264ES60 96264ES76 Recombinant Cynomolgus HGF R/c-MET Protein, His Tag, Ultra Low Endotoxin 25 μg 100 μg 500 μg Performance parameters Synonyms MET;oncogene MET;HGF R;HGF/SF receptor;AUTS9;cMET;Met (c-Met);RCCP2;SF receptor Species Cynomolgus Expression Sequence Glu25-Asn930 Accession A0A2K5UM36 Tag C-His Expression System HEK293 Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 69.9 kDa (β chain). Due to glycosylation, the protein migrates to 45-52 kDa (α chain) and 75-82 kDa (β chain) based on Bis-Tris PAGE result. Purity > 95% as determined by SDS-PAGE and SEC-HPLC Endotoxin Less than 0.01 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological activity ELISA Data 1:Immobilized Cynomolgus HGF R, His Tag at 5 μg/ml (100μl/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA . Reconstitution Method Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. Storage Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves,for your safety. Documents 96264_Manual_HB2026515 Show more