IL-18RAP, also known as IL-18Rβ or IL-1R7, is component of IL-18 receptor complex and its interactions with IL-18R1 mediate signal transduction initiated by IL-18 although it does not directly bind IL-18 in vitro. Specifications Cat.No. 96266ES25 / 96266ES60 / 96266ES76 Size 25 μg / 100 μg / 500 μg Components Name 96266ES25 96266ES60 96266ES76 Recombinant Cynomolgus IL-18RAP/IL-18 R beta Protein,His Tag,Ultra Low Endotoxin 25 μg 100 μg 500 μg Performance parameters Synonyms IL-18 R beta; ACPL; CD218b; CDw218b; IL-18R-beta; IL-18Rbeta; IL-18RAcP; IL-1R-7;IL-1R7;IL-1RAcPL;IL18RB;IL18RAP;IL-18R-beta;IL-18R-β;IL-18Rβ Species Cynomolgus Expression Sequence Phe20-Arg356 Accession A0A2K5VDC3 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 39.47 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result. Purity > 95% as determined by SDS-PAGE and SEC-HPLC Endotoxin Less than 0.01 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological activity ELISA Data 1:Immobilized Cynomolgus IL-18RAP, His Tag at 0.2 μg/mL (100μl/well) on the plate. Dose response curve for Anti-IL-18RAP Antibody, hFc Tag with the EC50 of 3.5 ng/mL determined by ELISA. Reconstitution Method Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. Storage Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves,for your safety. Documents 96266_Manual_HB2026515
