Recombinant Cynomolgus IL-2 R beta/CD122 Protein, His Tag, Ultra Low Endotoxin _ 96268ES

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IL-2 R beta is a member of the cytokine receptor superfamily . Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal pe ptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Specifications Cat.No. 96268ES25 / 96268ES60 / 96268ES76 Size 25 μg / 100 μg / 500 μg Components Name 96268ES25 96268ES60 96268ES76 Recombinant Cynomolgus IL-2 R beta/CD122 Protein,His Tag,Ultra Low Endotoxin 25 μg 100 μg 500 μg Performance parameters Synonyms CD122;IL-15 R beta;IL-2 R beta;IL-2R subunit beta;IL2RB;P70-75;p75;RP5-1170K4.6;IL-15 R β;IL-2 R β;IL-2R subunit β Species Cynomolgus Expression Sequence Ala27-Asp239 Accession Q38J85 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 25.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. Purity > 95% as determined by SDS-PAGE and SEC-HPLC Endotoxin Less than 0.001 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological activity SPR Data: Cynomolgus IL-2 R beta, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.17 μM as determined in SPR assay . Reconstitution Method Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. Storage Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves,for your safety. Documents 96268_Manual_HB2026515 Show more