ACEA Biosciences RT-CES xCELLigence System – W200 Analyzer & 16× RT-CIM E-Plate Station (MPN: 100016-001) The ACEA Biosciences 100016-001 (now under the Agilent Technologies portfolio) is a high-performance, label-free microelectronic cell sensor system designed for real-time cellular analysis (RTCA). The system pairs the compact W200 RT-CES (Real-Time Cell Electronic Sensing) Analyzer with a specialized 16× RT-CIM E-Plate Cradle Station. Utilizing proprietary microelectrode impedance technology, this system enables non-invasive, continuous monitoring of cell proliferation, morphology changes, attachment strength, and cell invasion or migration processes without the need for destructive fluorescent or radioactive labeling reagents. Technical Performance and Design Impedance-Based Cell Index Tracking: Features integrated gold microelectrodes patterned onto the bottom of specialized E-Plates. As cells attach and spread across the electrode array, they alter the electrical impedance, generating a dynamic, multi-frequency "Cell Index" value in real time. Dual Functional Assay Horizons: The system interfaces natively with both standard 16-well E-Plates (for routine cell proliferation, cytotoxicity, and cell-adhesion assays) and dual-chamber CIM-Plate 16 modules (for automated cell migration and invasion profiling). Incubator-Safe Station Footprint: The 16× RT-CIM station is engineered with specialized moisture-resistant alloy casings, allowing it to be placed directly inside standard CO_2 incubators under high-humidity conditions (98% RH} while routing data externally to the analyzer module via flat ribbon lines. Label-Free, Non-Invasive Diagnostics: Eliminates endpoint-only assays and toxic chemical dyes. Because the low-voltage output test signal ($22\text{ mV RMS}$) is completely harmless to living tissue, cells remain biologically intact for downstream endpoint validations, Western blots, or genomic extractions. Product Specifications Catalog Number / MPN: 100016-001 Manufacturer / Brand: ACEA Biosciences / Agilent Technologies (xCELLigence® Line) System Configuration: W200 RT-CES Core Analyzer + 16-Well RT-CIM E-Plate Station Plate Capacity: One 16-well E-Plate, E-Plate VIEW 16, or CIM-Plate 16 per run Measurement Frequency Range: Multi-frequency switching (10 kHz, 25 kHz, and 50 kHz capabilities) Impedance Repeatability Metric: Accurate to within ±1.5% under stable climate profiles Communication Interface: RS-232 / USB Virtual Serial mapping at 57,600 bits/second Power Requirements: Universal 100–240 VAC, 50/60 Hz autosensing input Key Benefits & Applications Cancer Cell Invasion & Migration: The gold standard framework for tracking the kinetic movement of metastatic cancer lines through simulated extracellular matrices in Transwell-style assays. In Vitro Cytotoxicity & Drug Screening: Perfect for charting the exact time-dependent toxicity profile of candidate small molecules or immunotherapies without missing early-onset apoplectic events. Receptor-Mediated Cell Signaling: Captures rapid, transient morphological adjustments triggered by G-protein coupled receptors (GPCRs) or growth factor signaling pathways within seconds of ligand addition. Viral Cytopathic Effect (CPE) Kinetics: Utilized by virology research cores to continuously plot viral infection kinetics and neutralizer antibody efficacy curves without requiring plaque staining assays. Used. Excellent condition. All product names, logos, and brands are property of their respective owners.
