Description Actin, Alpha Skeletal Muscle, Rabbit Protein is an ultra-pure Actin protein in the alpha-skeletal muscle isoform, purified from rabbit striated muscle. The purification method used (according to Spudich & Watts) results in a highly purified protein with a molecular mass of 43,000 dalton. Summary Actin is a muscle protein localized in the I band of the myofibrils; acting along with myosin, it is responsible for contraction and relaxation of muscle. Each actin protomer binds one molecule of ATP and has one high affinity site for either calcium or magnesium ions, as well as several low affinity sites. Actin exists as a monomer in low salt concentrations, but filaments form rapidly as salt concentration rises, with the consequent hydrolysis of ATP. It occurs in globular (G-actin) and fibrous (F-actin) forms. Actin is found in all eukaryotic cells (except for nematode sperm). Actin is one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Its other functions include cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. CAS Registry No. 9064-37-3 Other Names Alpha-actin-1, ACTA1 gene product, actin alpha isoform, UniProtKB - P68135 (ACTS_RABIT) Source Rabbit muscle. Formulation Sterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized from a 1 mg/ml solution containing 10 mM Tris/HCl buffer pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 1 mM DTT and 0.5% (w/v) SDS. Purity Greater than 98.0%. Reconstitution It is recommended to reconstitute the lyophilized Actin in sterile 18MΩ-cm H2O not less than 1 mg/ml, which can then be further diluted to other aqueous solutions. Shipping At ambient temperature. Upon receipt, store the product at the temperature recommended below. Storage/Expiration Lyophilized Actin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Actin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Usage This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive. Resources Product Datasheet (PDS) Related Products Actin, Alpha Cardiac Muscle 1, Native Protein (Bovine Heart)
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